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Two-colour computerised perimetry is a technique developed for assessing cone- and rod-function at fixed background luminances in retinal disease. However, the state of adaptation during testing is unknown but crucial in the interpretation of results. We therefore aimed to determine the adaptational state of rod- and cone-mechanisms in two-colour perimetry. Sensitivity to 480nm (blue) and 640nm (red) Goldmann size V targets was determined for 10 normal subjects aged 16 to 46years at 17 locations in the central 60 degrees of the visual f