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Particularly Cu and Zn have become attractive, due to their paid down ecological and security problems compared to Ni. Co and Zn are far more selective than Ni and Cu. This increased selectivity comes during the cost of weaker binding. In this work, the influence of ligand option on protein purity after IMAC had been assessed by several practices, including peptide mapping. His-taggerding to ligand and by steel ions, with Zn and Co forming one cluster and Ni and Cu another. The co-elution
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