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aureus strains. The IMSA assay had high specificity compared with loop-mediated isothermal amplification (LAMP) it confirmed the presence of all 7 Staph. aureus strains, and we found no false-positive results for the 12 non-Staph. aureus strains. The lower limit of detection for the IMSA assay was 1 × 102 cfu/mL, 10-fold more sensitive than the results obtained using LAMP. We also successfully applied the IMSA assay to confirm the presence of Staph. aureus in milk samples of cows with mastitis, and the results were consistent with those