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As verified by fluorescence spectra and UV-Visible spectroscopy. Circular dichroism (CD) and Ultraviolet absorption investigations additionally demonstrated which binding of putrescine to acid phosphatase resulted in microenvironmental variants across the protein . Consequently, putrescine cause alterations in its framework and purpose. The outcome, therefore, revealed the effect of putrescine ended up being due to the kosmotropic specs. Molecular dynamics simulation and Molecular doc