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To date, microRNAs (miRs) carried in extracellular vesicles (EVs) in response to exercise have been studied in blood but not in non-invasively collectable body fluids. In the present study, we examined whether six exercise-responsive miRs, miRs-21, -26, -126, -146, -221, and -222, respond to acute endurance exercise stimuli of different intensities in sweat. We investigated the response of miRs isolated from sweat and serum EVs to three endurance exercise protocols (1) maximal aerobic capacity (VO ), (2) anaerobic threshold (AnaT), and (