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We examined the periodical structure and permeability of lipid movies consists of ceramides (Cer; specifically, N-lignoceroyl 6-hydroxysphingosine, CerNH24, and N-lignoceroyl sphingosine, CerNS24), lignoceric acid (LIG; 24, and cholesterol levels (Chol). X-ray diffraction experiments showed that the CerNH24-based samples form either a quick lamellar phase (SLP, d ∼ 5.4 nm) or a medium lamellar phase (MLP, d = 10.63-10.78 nm) with respect to the annealing conditions. The suggested molecular arrangement associated with MLP pr