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Background Preschool years are an important time for shaping healthy eating behaviors. Childcare centers can be a venue for policy change for broad and sustained positive impact on healthy eating environment. The objectives of this study were to assess how self-reported current practices align with updated statewide childcare center licensing regulations in Colorado, US, and to explore correlates of adherence. Methods Using a post-test only study design, a survey was sent to all full-day, licensed childcare centers in Colorado (N = 1,398