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24.7 (p0.001), 37.55.2 (p0.001), and 6.44.0 dB (p0.05) in the MST-CBE imaging, respectively. Correlation coefficient and bias between cross-sectional dimensions of the ablation zones measured in tissue sections and estimated from MST-CBE were up to 0.91 (p0.001) and -0.02 mm2, respectively. The MST-CBE approach can monitor the detailed changes within target tissues and effectively characterize the dimensions of the ablation zone during CBUS energy deposition. The MST-CBE approach could be practical for improve