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The results obtained indicated that the correlation coefficients were linear ≥0.9934. The values of LLOQ in the methanol, PVA 1%, PBS, TSB, and epidermis muscle were 7.20 µg/mL, 4.40 µg/mL, 8.18 µg/mL, 387.48 µg/mL, and 7.27 µg/mL, respectively. The accuracy and precision of the evolved method were prominent. These methods had been effectively applied to quantify the total amount of CHL in microparticle and microneedle system in fluid and tissue skin illness. The effect showed the larg