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This study provides significant insights into the neurological effects of GFNs, and suggests that G-NH2 is not as safe as reported in many previous studies. The neurological effect of GFNs over longer term exposure should be considered in future studies.In the search for new co-crystal forms, many studies only consider one method of co-crystallisation which may lead to incorrect results. In this work, we demonstrate the efficiency of applying multiple experimental and virtual screening methods for a more comprehensive search for co-crystals of acetazolamide