https://www.selleckchem.com/pr....oducts/ngi-1ml414.ht
For purification of the thermophilic his-tagged ALDHTt, heat treatment, immobilized metal affinity chromatography (IMAC), and gel filtration chromatography were used. The enzyme activity assay was performed via UV-Vis spectrophotometry, monitoring the production of reduced nicotinamide adenine dinucleotide (NADH). Graphical abstract Flow chart outlining the steps in ALDHTt expression and purification, highlighting the approximate time required for each step.In most biomedical labs, researchers gather metadata (i.e., all details about
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