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This study identifies the role and significance of heat treatment parameters on blood compatibility and hemostatic performances. Bioactive nanomaterials step-annealed at 550 °C and 600 °C enhances the biocompatibility due to the liberation of nitrate content. This also develops the anisotropic structures such as needle-like and rod-like appearances that positively improve the erythrocyte compatibility. Different stable crystalline phases such as NaCaPO4 , Na2 Ca2 Si3 O9 and Na1.8 Ca1.1 Si6 O14 were observed for all the bioactive materia