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The results of PA pretreatment from the structural traits of SCB were also studied plus the digestibility of pretreated SCB was also evaluated by dark fermentative hydrogen production with an enriched anaerobic cellulolytic microbial consortium MC1. The hydrogen manufacturing increased by 195.5per cent (considering initial SC in addition to abundance of principal hemicellulose-degradation genus Thermoanaerobacterium increased from 23.8% to 40.2per cent as a result of