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The release profile at 37 °C showed that ω-3 PUFAs were slowly released at pH 2 but was rapidly released at pH 7.4 from Lf nanoemulsions. Moreover, MTT assay revealed that 2% (w/w) Lf nanoemulsions with 12.5 μg mL-1 ω-3 PUFAs were not cytotoxic to Caco-2 cells. Nanoemulsions with high physical and chemical stability were selected and dried by two different methodologies freeze-drying and nano spray-drying. ATR-FTIR spectroscopy, Raman spectroscopy and Circular Dichroism (CD) showed Lf structural changes after the drying processes. Thi