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The transformants had been screened with polymerase sequence effect and Western blot analyses. Enzyme-linked immunosorbent assay (ELISA) confirmed the binding associated with purified anti-HER2 VHH-FcK to your HER2-positive breast cancer cellular range, SK-BR-3. Migration assay outcomes confirmed anticancer activity regarding the plant-derived anticancer camelid single string antibody. Taken together, we confirmed the likelihood of utilizing an