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Ultrasonic backscatter techniques may offer a useful approach for detecting changes in bone caused by osteoporosis. The goal of the present study was to investigate how bone mineral density (BMD) and the microstructure of human cancellous bone affect three ultrasonic backscatter parameters that have been identified as potentially useful for ultrasonic bone assessment purposes apparent integrated backscatter (AI, frequency slope of apparent backscatter (FSA, and frequency intercept of apparent backscatter (FIA. Ultrasonic measureme