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The two major causes of microcytic anemia are iron deficiency and thalassemia. In clinical practice, in some cases, differentiation of microcytic and hypochromic anemia become difficult on the basis of traditional red cell parameters. This study primarily aimed to evaluate the usefulness of red cell distribution width (RDW), immature reticulocyte fraction (IRF), and reticulocyte hemoglobin equivalent (Ret-He) in the discrimination of alpha thalassemia, beta thalassemia, and latent iron deficiency. In this retrospective study laboratory da