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The isolated heparin samples were also analyzed by 1H NMR spectroscopy to check the possible impurities, and the results show the presence of chondroitin sulfate and dermatan sulfate, as is the case for the heparin eluted from the commercial resin. Furthermore, the effects of some experimental variables including the adsorbent dosage, pH, time, and recycling on heparin adsorption were studied, and the results show that these resins can be used for efficient recovery of heparin.The main impetus of vascular tissue engineering is clinical transla