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Serial femtosecond crystallography (SFX) with X-ray free-electron lasers (XFELs) has proven highly successful for structure determination of challenging membrane proteins crystallized in lipidic cubic phase; however, like most techniques, it has limitations. Here we attempt to address some of these limitations related to the use of a vacuum chamber and the need for attenuation of the XFEL beam, in order to further improve the efficiency of this method. Using an optimized SFX experimental setup in a helium atmosphere, the room-temperature s

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Take Your Treadmill to the Next Level With a Treadmill With an Incline of 12 When you're ready to take your treadmill workouts to the next level, you should consider the treadmill with the highest incline of 12. This is a more difficult exercise that feels like walking up hills. It's best to get used to this by gradually increasing the slope over time, advises Burgau. Start with a low slope and take shorter walks, then build up to longer walks. What is an Incline Treadmill? You can raise the