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0002 ng μL for R. pretiosus and 0.002 ng μL for L. flavobrunneum. In addition, the LOD for mixed muscle tissues was 0.1 g kg . Oilfish- and escolar-derived components were detected in 12 of the 70 commercial samples, a result that is consistent with the classic DNA barcoding test results. The duplex real-time PCR method developed can be used to detect oilfish and escolar specifically, sensitively and accurately; it provides a good technical support for the identification of authentic aquatic products. © 2020 Society of Chemical Industry.