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In comparison, no infectious parasites had been detected in the amustaline/GSH-treated test after 4 weeks of culture. CONCLUSION A robust level of P. falciparum inactivation was attained in WB making use of amustaline/GSH therapy. Parasite log decrease had been 5.7 log10 TCID50 per mL. Growth of such a pathogen reduction system might provide a chance to decrease the threat of TT malaria and improve blood availability. © 2020 The Authors. Tra