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Scrotal insulation (SI) for 48 hour had been applied to Brahman (Bos indicus) bulls. Semen was gathered at 3-day intervals from before, until 74 days post-SI. The changes in sperm morphology and motility following SI were similar to formerly reported and differences were detected in measures of sperm chromatin conformation as soon as 8 days post-SI. New proteins places, when you look at the SPP two-dimensional (2-D) ties in, had been apparent when you compare pre-SI wit