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Herpesvirus infections associated with a range of clinical findings are widespread in free-ranging and captive Australian marsupials. We report on herpesviruses identified by virus neutralization and PCR in free-ranging and captive Lumholtz's tree-kangaroos (Dendrolagus lumholtzi). Herpesvirus has not been confirmed previously by DNA testing in tree kangaroos. Virus neutralization testing for alphaherpesviruses MaHV-1 and MaHV-2 was positive on 4/10 captive and 0/35 free-ranging tree-kangaroo samples tested. A novel gammaherpesvirus wa
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