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6% and 1.2% mismatched sequences, respectively, although most of these mismatches did not affect the amplification efficiency, with the exception of the 3' end of the NIID-N2 forward primer. These findings indicate that the previously developed NIID-N2 assay remains suitable for the detection SARS-CoV-2 with support of the newly developed NIID-S2 set.We aimed to determine the predictors of intensive care unit (ICU) admission or death in patients with Coronavirus Disease 2019 (COVID-19) pneumonia. This retrospective and single-center study