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The findings from this study may lead to the formulation of bioherbicides that will improve human and environmental health.Iron is a fundamental element required by most of organisms, including Brucella. Several researchers have suggested that the iron response regulator (irr) gene and rhizobial iron regulator (rirA) regulates the iron acquisition of Brucella abortus (B. abortus), which influences the heme synthesis and virulence of this pathogen. However, little is known about another Brucella species, Brucella melitensis (B. melitensis).