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The derivative diffuse reflectance UV-vis-NIR spectroscopy combined with the multivariate methods are utilized for the discrimination and classification of the soil samples collected from the north-western part of India. The acquired spectra reveal the presence of different organic and inorganic minerals such as humic acid, fulvic acid, hematite, etc. in varying amounts. The differentiation/segregation among soil samples is achieved by peak comparison and chemometric methods like clustering algorithm and principal component analysis (P