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The most frequently mutated genes were (five cases), (four cases), (three cases), (three cases), (two cases), (two cases), (two cases) and (two cases). We developed a targeted NGS approach to identify tumor-specific alterations in ctDNA of neuroblastoma patients. Our results show the reliability of our approach to generate genomic information which can be integrated with clinical and pathological data at diagnosis. We developed a targeted NGS approach to identify tumor-specific alterations in ctDNA of neuroblastoma patients. Our result