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Electrochemical detection is the simplest analytical tool to be integrated into digital microfluidics (DMF). It offers the advantages of small size, with detector electrodes incorporated into the device by patterning, and high compatibility with portable analytical instruments. Indium tin oxide (ITO) coated on glass has been commonly used for the top plate of DMF due to its good conductivity and transparency. However, instability and the low current response of ITO electrodes patterned on glass hindered their application fo

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25-hydroxyvitamin D (VD) effects on lung function and immune-modulation might affect respiratory syncytial virus (RSV) infection outcomes. We aimed to assess VD levels on admission and their association with life-threatening RSV disease (LTD). A prospective cohort study was conducted during 2017-2019. Previously healthy infants aged 12 months, hospitalized with a first episode of RSV infection, were enrolled. LTD was defined by need for intensive care and ventilatory support. Serum VD levels 20ng/mL were categorized as deficient,