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Results verified that this method is applicable for Jurkat cells. Sample flow maintains its stability without clogging up even at high sample concentration. Focusing still works at flow velocity over 100 μl/min. All these results certify this acoustic particles focusing method can enhance the performance of hydrodynamic flow cytometer by minor modification.Two analytical methods were developed using electrochemical and spectrometric techniques for the simultaneous determination of endocrine disruptors triclosan and methylparaben in the m