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n compared to the traditional protocol. Using only commercial preparations may be helpful for widespread application of RCA. Clinicaltrials.gov. NCT02663960 . Registered 26 January 2016. Clinicaltrials.gov. NCT02663960 . Registered 26 January 2016. Cattle are ideally suited to investigate the genetics of male fertility. Semen from individual bulls is used for thousands of artificial inseminations for which the fertilization success is monitored. Results from the breeding soundness examination and repeated observations of semen quality c