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In summary, this novel set of tools allows to characterize mRNA distribution in astrocytes and microglia in physiological or pathological settings. © 2020. Published by The Company of Biologists Ltd.All cells establish and maintain an axis of polarity that is critical for cell shape and progression through the cell cycle. A well-studied example of polarity establishment is bud emergence in yeast, which is controlled by the Rho GTPase Cdc42p. The prevailing view of bud emergence does not account for regulation by extrinsic cues. Here, w
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