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The results of this study confirmed that the level of treatment considerably affected the costs of WWTPs. Moreover, the CFs were separately derived for tertiary treatment including A2O with and without digester. The CFs obtained in this study are of utmost importance to be used in the economic evaluation of the planned WWTPs and in the management of existing ones.Large quantities of engineered nanoparticles (ENPs) have emerged on the European market with the rapid development of nanotechnology, however knowledge of potential health risks