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By virtue associated with the favorable optical properties of upconversion nanoparticles (UCNPs), the latter is done by using two-color UCNPs (Er-UCNPs and Tm-UCNPs) with negligible spectral overlaps. Because of the help associated with dual key strategies, we fabricated complex microbeads labeled a UCNPs-miRNAs-microbead sandwich construct by a one-step nucleic acid hybridization procedure then received uniform terrace peaks when it comes to automated and simultaneous quantitative determination of mi
