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Despite differences in economy, cyclists climb in seated and standing positions. Prompted by gaps in research, we compared VO2 and heart rate (HR) (Study 1), muscle activation (Study 2) and breathing and pedaling entrainment (Study 3). Subjects rode their bicycles on a treadmill in seated and standing positions. In Study 1, VO and HR of four male cyclists (21.3 ± 1.7 yrs; 69.1 ± 6 ml/kg/min) were collected, alternating positions every 5 minutes for 20 minutes (8 mph, 8% grade). In Study 2, muscle activations of eight male cyclists (24 ±
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