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The contribution to health-based reference values and benefit-risk quotients indicated that the health benefits of consumption of fish from lake Trasimeno outweigh the potential risks.The surface energy and bonding coefficient of asphalt are important factors that affect the adhesion performance of asphalt/aggregate. In this study, the micro-bee-like-structure of asphalt and force curves between the microscope-probe and asphalt were measured via atomic force microscopy (AFM). To investigate the influence of asphalt properties on micro-adhesion of asphalt, f