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Energy-efficient process for water desalination and organic dye separation is urgently needed to meet the dramatically increasing demand for fresh water globally. Nb2CTx MXene, as one type of 2D transition metal carbides (TMDCs) family, has attracted tremendous research interest in the fields of separation technology over the past decade. However, it has been challenging to fabricate surface-modified Nb2CTx MXene nanosheet films with superior performance for desalination and separation applications. Herein, we report a novel, facile and