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Chemical expansion regarding the plasma membrane or osmotic inflammation had no effects. However, osmotic shrinking of neutrophils inhibited both cell dispersing and phagocytosis. Triggering a Ca2+ sign in osmotically shrunk cells (by IP3 uncaging) evoked tubular blebs instead of full cell spreading. Phagocytosis was stopped during the phagocytic glass stage by osmotic shrinking induced following the phagocytic Ca2+ signalling. Restoration of isotonicity manage