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MacConkey Agar, a selective and differential medium, distinguishes between lactose fermenters and non-fermenters among gram-negative bacteria. Its crystal violet and bile salts inhibit gram-positive growth, fostering a pink coloration in lactose fermenters due to acid production and neutral red pH indicator. Non-fermenters appear colorless. Developed by Alfred Theodore MacConkey in the 20th century, this agar aids in identifying enteric pathogens in clinical and environmental samples, essential for diagnosing infections and monitoring hygiene in food and water industries.

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MacConkey Agar - Composition, Principle, Preparation - TM Media
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MacConkey Agar - Composition, Principle, Preparation - TM Media

MacConkey agar and broth have been recommended for use in the microbiological examination of foodstuffs and for direct plating or inoculation of water samples for coliform counts.