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To explore factors associated with a high vaginal GBS load during labor considering (1) the recto-vaginal GBS load at 35-37 weeks' gestation determined by culture and (2) the vaginal GBS colonization determined by a polymerase chain reaction (PCR) assay during labor. From an unselected cohort of 902 pregnant women, we obtained (1) recto-vaginal swabs for culture of GBS at 35-37 weeks' gestation (GBS ), (2) vaginal swabs for GBS PCR detection at labor (PCR ), and (3) vaginal swabs for culture of GBS at labor (GBS ). The GBS load was cl