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However, no significant difference was found between the isoluminant and grayscale groups. The correlation coefficient between the original TNO and isoluminant groups was 0.952 (Spearman's rho, P less then 0.001; 95% confidence interval (CI), 0.901-0.988), while that between the original and grayscale groups was 0.771 (Spearman's rho, P less then 0.001; 95% CI, 0.550-0.916). Anaglyph glasses played an important role in determining the stereoacuity values with the TNO stereo test, and the results were overestimated when compared with th