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The human placenta is considered a biological waste, thus it is a great source of extracellular matrix (ECM) proteins. The human chorion membrane (HCM) is a membrane that composes the human placenta and is constituted by collagens type I, II, IV, V and VI, fibronectin and laminin. To the best of our knowledge, the potential of HCM alone is largely unexplored as a substrate to be used in tissue engineering and regenerative medicine. In this work, we describe, for the first time, the process and method to decellularize the chorion membrane