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Objective The aim of this in vitro study was to investigate the possible interactions between photon-induced photoacoustic streaming (PIPS™)-activated oxidizing agents and 2% chlorhexidine digluconate. Background data There is no information about the safety of laser-activated oxidizing agents in combination usage with chlorhexidine gluconate. Materials and methods Groups were designed as follows G1 98% para-chloroaniline (PCA); G2 2% chlorhexidine (CHX); G3 5.25% sodium hypochlorite (NaOCl) +2% CHX; G4 5.25% NaOCl (30 sec PIPS activate