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ndothelial cells. These results demonstrate that the size and duration of shear stress can affect the morphology and arrangement of endothelial cells. The commonly used evaluation indicators for studying the effect of shear stress on the morphology of endothelial cells, including area, perimeter, long axis diameter, short axis diameter and orientation angle, etc., had no significant statistical significance, while the intercellular space characteristics including the junction length per unit area and the triple points per unit area can be