https://www.selleckchem.com/pr....oducts/epibrassinoli
Two pure compounds with antityrosinase activity were isolated by silica gel 60 column chromatography (SG60CC) and high performance liquid chromatography (HPLC), and their chemical structure deduced by Nuclear Magnetic Resonance (NMR) analysis. Results The DCM partitioned extract of SBP (DCMSBP) had an antityrosinase activity (IC50, 159.4 μg/mL) and was fractionated by SG60CC, providing five fractions (DCMSBP1-5). The DCMSBP5 fraction was the most active (IC50 = 18.8 μg/mL) and further fractionation by HPLC gave two active fracti