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Herein, we employ this workflow to examine farnesol reduction using a codon-saturation mutagenesis library at the Leu377 siteof SaGGR. We show this TLC-based screen can distinguish between fourfold differences in enzyme activity for select mutants and validated those results by GC-MS. With appropriate quantitation methods, this workflow can be used to screen polyprenyl reductase activity and can be readily adapted to analyze broader catalyst libraries whose products are amenable to TLC analysis. With appropriate quantitation methods, thi
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