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To minimize the impact caused by various sampling protocols, the use of a volume gradient trail experiment and a unified mesh size of 60-100 μm for the initial concentration are recommended according to the results of this review. Given the limited knowledge regarding the vertical transport of MPs in the water column, harmonized sampling methods should first be developed. The mechanisms of this process can be separately considered for different water bodies, such as freshwater systems, coastal waters, and pelagic zones. The presence of t