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The aims of this study were to investigate the effects of probiotics and antibiotics on microbial composition, short chain fatty acids (SCFAs) concentration and free fatty acid receptor 2/3 (FFAR2/3) expression in boiler chickens. A total of 150 1-day-old male broilers were randomly allocated into three groups, control (CON) group, probiotics (P group and antibiotics (AT group. Results indicated that PB improved the average body weight from 1 to 21days and feed intake from 21 to 42days (P0·05), while ATB improved the feed effici