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Conclusion The expression of CHKA in glioma tissue is significantly higher than that in the normal brain tissue, and knockdown of CHKA gene inhibits the proliferation, invasion and migration of glioma cells. It suggests that CHKA may be related to the occurrence and development of gliomas.Objective To observe the changes of the acetylation of lysine 9 on histone H3 (H3K9) and H3K14 in the different brain regions during rapid eye movement after sleep deprivation (SD) in rats. Methods Modified multiple platform was used to establish the SD