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Earthworms as ecosystem engineers partially improve soil properties by egesting casts. Our previous study confirmed that soil pollution affects the physico-chemical properties of earthworm casts. It is still unclear whether the biological properties (e.g. cellulase, urease, and acid and alkaline phosphatase activities, as well as microbial biomass carbon) of casts are affected by foreign substances in soil. The present study aimed to investigate the effect of phenanthrene (PHE) on the biological characteristics of earthworm (Eisenia feti