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Additionally, adding microfiber provided more sites for microbes and increased the total number of microbes in unsaturated zone. The addition of PBS in the saturated zone obviously improved the denitrification efficiency with the total nitrogen (TN) removal rate raising from 20.6 ± 4.0% to 90.4 ± 2.7%, which excellently solved the problem of poor denitrification efficiency caused by low ratio of carbon to nitrogen (C/N). In conclusion, the association of microfiber and PBS in partially unsaturated constructed wetland finally accomplished